Acetoin production enhanced by manipulating carbon flux in a newly isolated Bacillus amyloliquefaciens
2013
Zhang, Yanjie | Li, Shubo | Liu, Liming | Wu, Jing
A new strain, FMME044, exhibited a remarkable ability to synthesize acetoin and was identified as Bacillus amyloliquefaciens. The following characteristics of enzyme activity were found: 2,3-butanediol was reverse transformed to acetoin upon depletion of glucose; lower agitation speeds favored 2,3-butanediol accumulation; and higher agitation speeds favored reverse transformation of 2,3-butanediol to acetoin. In order to enhance acetoin production by manipulating the carbon flux distribution, a two-stage agitation speed control strategy was proposed: during the first 24h, the agitation speed was set to 350rpm to achieve a high 2,3-butanediol concentration and then the speed was increased to 500rpm to reverse transform 2,3-butanediol to acetoin. Following this strategy, a high titer (51.2gL−1), yield (0.43gg−1), and productivity (1.42gL−1h−1) of acetoin were achieved. The results demonstrated that B. amyloliquefaciens FMME044 is a potential industrial strain for acetoin production.
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