A highly stable, sensitive, regenerable and rapid immunoassay for detecting aflatoxin B₁ in corn incorporating covalent AFB₁ immobilization and a recombinant Fab antibody
2013
Edupuganti, Soujanya Ratna | Edupuganti, Om Prakash | Hearty, Stephen | O’Kennedy, Richard
A highly robust immunoassay applicable for the detection of aflatoxin B₁ (AFB₁) using a Fab antibody fragment was developed. A key factor was the use of covalently immobilized AFB₁ which allowed an almost three fold increase in sensitivity, reduced assay time and regeneration with retention of binding capacity. Various factors that might affect the sensitivity of the assay such as pH, organic solvents, storage stability and wash stringency were critically evaluated. It was also demonstrated that the assay was applicable for determination of AFB₁ in corn samples at concentration within the European union regulatory limits.
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