Effect of process parameters on enhanced biohydrogen production from tequila vinasse via the lactate-acetate pathway
2019
García-Depraect, Octavio | Rene, Eldon R. | Diaz-Cruces, Víctor F. | León-Becerril, Elizabeth
In this study, a lactate-type fermentation entailing the consumption of lactate and acetate (lactate-acetate pathway) is proposed to deal with lactic acid bacteria (LAB) inhibition during the production of biohydrogen (bioH₂) from tequila vinasse. The effects of total solids content, substrate concentration, nutrient formulation and inoculum addition on bioH₂ production performance were investigated. Batch experiments were performed in a 3-L completely mixed reactor at 35 °C and pH 6.5–5.8. The lactate-acetate pathway mediated consistent bioH₂ production which was influenced by inoculum addition followed by substrate concentration, nutrient formulation and solids content. Maximum bioH₂ production rate (225 NmL/L-h) and yield (124 NmL/g VSₐddₑd) were achieved by removing suspended solids and enhancing nutrient content, respectively. Illumina sequencing-based analysis revealed a dominance of Clostridium in the inoculum, which together with LAB and acetic acid bacteria shaped a keystone cluster for avoiding LAB inhibition while ensuring consistent bioH₂ production performance.
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