Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer)
Hooft, W.F van | Hanotte, O. | Wenink, P.W. | Groen, A.F. | Sugimoto, Y. | Prins, H.H.T. | Teale, A.
The applicability of bovine autosomal micro-satellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel of seven African buffalo. Amplification was observed for 139 markers (83%), and 101 markers were studied further with 91 (90%) being polymorphic. The mean number of alleles per marker was 5(.)0 (SE = 0(.)2) and the mean heterozygosity per marker was 0(.)61 (SE = 0(.)03). Considering the overall high level of polymorphism, it was concluded that most bovine microsatellite markers are applicable in African buffalo.
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