Fluroxypyr- and triclopyr-specific enzyme-linked immunosorbent assays: development and quantitation in soil and water
1996
Johnson, B.D. | Hall, J.C.
Enzyme-linked immunosorbent assays (ELISAs) were developed to quantitate fluroxypyr [[(4-amino-3,5-dichloro-6-fluoro-2 -pyridinyl)oxy]acetic acid] and triclopyr [[(3,5,6-trichloro-2-pyridinyl)oxy]acetic acid] in soil and water. The linear working range of the fluroxypyr ELISA was from 0.1 to 10 ng.mL-1 with a limit of detection (LOD) of 0.1 ng.mL-1 and an IC50 value of 1.6 ng.mL-1. The linear working range of the triclopyr ELISA was from 0.1 to 5 ng.mL-1 with a LOD of 0.1 ng.mL-1 and an IC50 value of 1.7 ng.mL-1. Cross-reactivity to selected pyridine metabolites and agrochemicals was determined. Significant cross-reactivity (within the linear working range of the ELISA) using the fluroxypyr ELISA was found only to the metabolite 4-amino-3,5-dichloro-6-fluoro-2 -methoxypyridine. Significant cross-reactivity using the triclopyr ELISA was found only to the auxinic herbicide 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The ELISAs accurately estimated fluroxypyr and triclopyr in water at concentrations as low as 0.1 ng.mL-1. Analysis of two different soil types with different textures (clay loam and sandy clay loam) required cleanup procedures using filtration and solid phase extraction to accurately estimate fluroxypyr and triclopyr concentrations.
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