Protein concentrate from normal and high-lysine corns by alkaline extraction: preparation
Wu, Y.V. | Sexson, K.R.
An alkaline extraction process was developed to produce protein concentrates and starch from ground normal and high-lysine corns. Optimum extraction of protein occurred at pH 11.7 in 0.1N NaOH with 150 g corn/900 ml solvent. The corn was extracted twice with NaOH solutions. After centrifugation, each alkaline extract was adjusted to pH 4.7 to recover the protein as a precipitate. Bran was removed from starch and protein by screening the second alkaline dispersion, and the protein and starch were separated by centrifugation. The protein content (N × 6.25) of the concentrate varied between 63 and 71% and accounted for 52-63% of the total corn protein. High-lysine corn yielded more concentrate than normal corn, as well as more starch.
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