Molecular Evaluationof Genetic Variability in Blackgram Genotypes using RAPD Markers
2012
Suneja, Yadhu | Kaur, Satvir | Gupta, Anil Kumar | Narinder Kaur,
Blackgram, (Vigna mungo L. Hepper), with a haploid chromosome number of 11, is a self pollinating diploid (2n=2x=22) and India is its largest producer and consumer in the world. However, due to relatively poor genetic base of present day cultivars, its productivity is declining. In the present investigation, te n recommended cultivars and twenty advance breeding lines of blackgram were characterized at the molecular level using nine RAPD primers. The per cent polymorphism ranged from 50 to 100 per cent. The average polymorphic information content (PIC) was 0.702, varying from 0.50 to 0.85. Jaccard’s similarity coefficient ranged from 17.2% to 94.4% among the pair of genotypes, the highest being observed between Mash1-1 and Mash-114, and the lowest between Shekhar-2 and KUG-521. Shekhar-2 appeared quite distinct in the grouping based on principal component analysis. A dendrogram constructed on the basis of UPGMA clustering method revealed two major clusters. Cluster-I constituted ten r ecommended cultivars, whereas all advance br eeding lines grouped together into cluster-II. Cluster-II could be further differentiated into four sub clusters-B, C, D and E. Genotypes namely, KUG-230, KUG-386, KUG-391 and KUG-508, which had higher protein content, fell into sub cluster-B. Clustering of genotypes on the basis of RAPD data showed high concordance with categorization bases on biochemical diversity evaluated in terms of their nutritional constituents and antinutritional factors. The information obtained can be used by the plant breeders to choose suitable parents to produce genetically diverse lines.
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