New approach to water-soluble carbohydrate determination as a tool for evaluation of plant cell wall degrading enzymes
1993
Slominski, B.A. | Guenter, W. | Campbell, L.D.
Studies were conducted to develop a rapid method for the determination of total water-soluble carbohydrates in feedstuffs. Conditions required to result in the satisfactory hydrolysis of plant carbohydrates using HCl without substantial decomposition of constituent sugars were established. The method was validated by recovery studies utilizing mixtures of pure carbohydrates and by comparison of results with those determined by individual analysis of soluble polysaccharides in canola samples. In addition, the total water-soluble carbohydrate content of a number of different feedstuffs was determined, and the application of the method in the evaluation of the efficacy of various commercially available carbohydrase-like enzyme preparations on the hydrolysis of canola meal cell wall polysaccharides was demonstrated.
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