Characterization of genetic diversity and population structure of Moroccan lentil cultivars and landraces using molecular markers
2019
Mbasani-Mansi, Joseph | Ennami, Mounia | Briache, Fatima Zahra | Gaboun, Fatima | Benbrahim, Nadia | Triqui, Zine El Abidine | Mentag, Rachid
Knowledge of genetic diversity and population structure is a crucial step for an efficient use of available material in a plant breeding program and for germplasm conservation strategies. Current study undertakes an assessment of the genetic variations and population structure of Moroccan lentil including nine landraces and eight released varieties using sequence-related amplified polymorphism (SRAP) and random amplified polymorphism DNA (RAPD) markers. Results revealed that the two markers used have a good efficiency to assess genetic diversity in lentil. A total of 115 and 90 bands were respectively scored for SRAP and RAPD, of which 98.3% and 93.3% were polymorphic. The polymorphic information content values were 0.350 with SRAP and 0.326 with RAPD. Analysis of molecular variance based on the combined data sets of both markers revealed lower variations within (35%) than among (65%) landraces (PhiPT = 0.652), implying significant genetic differentiation between landraces. Principal coordinate analysis and the ascendant hierarchical classification clustered samples into groups that were consistent with the geographical origin of the cultivars. Population structure corroborated the main groups and confirmed the high differentiation among them. Moroccan lentil germplasm showed a wide genetic diversity that might be conserved and assessed for tolerance to biotic and abiotic stresses.
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Эту запись предоставил National Agricultural Library