Effects of post-emergent herbicides on <i>Trichoderma harzianum</i> , a potential biocontrol agent against <i>Sclerotinia sclerotiorum</i> in soybean cropping
2007
Rollán, M. C. | Mónaco, C. | Lampugnani, G. | Arteta, N. | Bayo, D. | Urrutia, M. I.
<i>Trichoderma harzianum</i> is a potential biocontrol agent against <i>Sclerotinia sclerotiorum</i> in soybean. Information is needed on the compatibility of this biocontrol agent and the post-emergent herbicides used in soybean cropping.Haloxyfop R Methyl (EC 10.4%), Glyphosate (SL 48%), Imazamox (WG 70%) and Imazethapyr (SL 10%) were evaluated for their effects on the mycelial growth of <i>T. harzianum</i> on <i>in vitro</i> agar plates. Glyphosate (2000 ppm), Imazethapyr (500 and 250 ppm) and Haloxyfop R Methyl (1000, 500 and 100 ppm) reduced the mycelial growth of <i>T. harzianum</i> . Imazamox had no effect at any concentration.Subsequently, all the herbicides were assessed for their effect on soil populations of <i>T. harzianum</i> . Greenhouse assays conducted with non-sterile soil inoculated with <i>T. harzianum</i> and a specific herbicide were sampled before pesticide application and after 30 days. The number of colony forming units per gram of soil (c.f.u./g of soil) was evaluated with a soil dilution technique using <i>Trichoderma</i> selective medium (TSM). No detrimental effect was revealed.
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