Developing an emulsion model system containing canthaxanthin biosynthesized by Dietzia natronolimnaea HS-1
2012
Gharibzahedi, Seyed Mohammad Taghi | Razavi, Seyed Hadi | Mousavi, Seyed Mohammad
An acceptable strategy to incorporate canthaxanthin (CX) as a natural colorant into products is by means of oil-in-water emulsions. The used CX in this study was produced by bacterium Dietzia natronolimnaea HS-1 using a batch bioreactor system. A central composite rotatable design–response surface methodology (CCRD–RSM) consisting of three-factored factorial design with five levels was applied for analysis of the results to obtain the optimal formulation of emulsions. Three independent variables including fenugreek gum (FG, 0.2–0.5%, w/w), coconut oil (CO, 6–10%, w/w), and CO/CX ratio (10:1–50:1) were transformed to coded values and second-order polynomial models was developed to predict the responses (p<0.0001). The studied independent variables were the stability, viscosity and droplet size properties such as volume-weighted mean diameter (D₄₃), specific surface area (Sᵥ) and polydispersity index (PDI) of emulsions. The 3-D response surface plot derived from the mathematical models was used to determine the optimal conditions. Main emulsion components under the optimum conditions ascertained presently by RSM: 50:1 CO/CX ratio, 0.49% (w/w) FG content and 6.28% (w/w) CO concentration. At this optimum point, stability, viscosity, D₄₃, Sᵥ and PDI were 90.6%, 0.0118Pas, 0.595μm, 12.03m²/ml and 1.380, respectively.
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