Phenolic-rich extracts from Willowherb (Epilobium hirsutum L.) inhibit lipid oxidation but accelerate protein carbonylation and discoloration of beef patties
Cando, Deysi | Morcuende, David | Utrera, Mariana | Estévez, Mario
An extraction procedure of phenolics from Willowherb (Epilobium hirsutum L.) was optimized; extracts were characterized in terms of phenolic content, composition, and in vitro antioxidant activity. Additionally, the effect of 50, 200, and 800 ppm Willowherb extracts on the oxidative stability (formation of thiobarbituric acid-reactive substances, α-aminoadipic, and γ-glutamic semialdehydes) of beef patties was studied. Acetone (50 %) extract, rich in ellagic acid, myricetin, hydroxybenzoic, and hydroxycinnamic acids, had the highest phenolic content compared to methanol (25, 50, 75, and 100 %), acetone (100 %), or water extracts and displayed intense in vitro antioxidant properties. Lipid oxidation levels in processed beef patties with added Willowherb extract remained as similar to that of 100 ppm gallic acid. A dose-dependent effect of Willowherb extract was observed on protein carbonylation and myoglobin oxidation. The lack of correlation between lipid and myoglobin oxidation and the underlying mechanisms involved in the pro-oxidant action of phenolics on meat proteins are thoroughly discussed.
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