Linolenic Acid Content of Soybean Seed Estimated with 2-Thiobarbituric Acid Test
1990
Bubeck, D. M. | Duvick, D. N. | Fehr, W. R. | Hammond, E. G.
The linolenic acid content in soybean [Glycine max (L.) Merr.] seed oil generally is determined by gas chromatography (GC). A more rapid method of comparing the linolenic acid content of soybean seed samples would be useful for screening a large number of lines in a soybean breeding program. The objective of this study was to determine if the 2-thiobarbituric acid (TBA) reaction could be used as a rapid method for determining the relative differences in linolenic acid content of soybean seed samples. Sixty-eight random F₂ plants from each of two populations were used to compare TBA scores with linolenic acid content as measured by GC. The correlations between TBA score and linolenic acid percentages were 0.71 for Cross 1 and 0.60 for Cross 2. The results indicated that the TBA test cannot be used as a substitute for GC analysis in evaluating soybean samples. However, the TBA test may be a useful method to rapidly eliminate genotypes with excessively high linolenic acid before GC analysis. Contribution from the Texas Agric. Exp. Stn., Journal Article TA24877. Funded in part by a grant from the U.S. Golf Assoc. Green Section.
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