Gibberellins in leaves of Alstroemeria hybrida: identification and quantification in relation to leaf age
1997
Kappers, I.F. | Jordi, W. | Maas, F.M. | Plas, L.H.W. van der
In alstroemeria (Alstroemeria hybrida), leaf senescence is retarded effectively by the application of gibberellins (GAs). To study the role of endogenous GAs in leaf senescence, the GA content was analyzed by combined gas chromatography and mass spectrometry. Five 13-hydroxy GAs (GA19, GA20, GA1, GA8, and GA29) and three non-13-hydroxy GAs (GA9 and GA4) were identified in leaf extracts by comparing Kovats retention indices (KRIs) and full scan mass sprectra with those of reference GAs. In addition, GA15, GA44, GA24, and GA34 were tentatively identified by comparing selected ion monitoring results and KRIs with those of reference GAs. A number of GAs were detected in conjugated form as well. Concentrations of GAs in alstroemeria changed with the development of leaves. The proportion of biologically active GA1 and GA4 decreased with progressive senescence and the fraction of conjugated GAs increased.
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