Identification of a naturally occurring Bean common mosaic virus recombinant isolate infecting Azuki Bean

Li, Y.; Cao, Y.; Wan, P.

Identification of a naturally occurring Bean common mosaic virus recombinant isolate infecting Azuki Bean

2016

Li, Y. | Cao, Y. | Wan, P.

The near complete nucleotide sequence of Bean common mosaic virus (BCMV) infecting azuki bean from China was determined to be 10047 nucleotides in length excluding the 3′-terminal poly (A) sequence. The isolate was designated as BCMV-Az and caused stunting, mosaic and crumpling on leaves of azuki plants. It contains a large open reading frame (ORF) flanked by a 131 nt 5′-untranslated region (UTR) and a 253 nt 3′-UTR and the putative polyprotein encoded by this large ORF is comprised of 3220 amino acid residues. Phylogenetic analysis showed that BCMV-Az clustered with strains isolated from different hosts and geographic origins, suggesting that it is a novel strain. Recombination analyses indicated that it was a recombinant originating from isolates R (AJ312437) and US10 (KF919299).

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