Identification of RAPD markers associated with the presence of B chromosomes in maize
1996
Gourmet, C. | Rayburn, A.L.
DNA isolated from Black Mexican sweet maize populations segregating for the presence of B chromosomes was used for PCR amplifications of 320 RAPD primers. Seventeen primers produced detectable polymorphisms between populations known to carry B chromosomes and populations devoid of B chromosomes. To ensure that the primers which generated a polymorphic band were indeed identifying B chromosomes, five Arizona Indian corn populations, three tetraploid corn populations and two High Loss corn lines differing in their B chromosome composition were analysed. Four primers were selected to screen these maize populations. Root tip counts were performed on the lines to confirm the association of the RAPD markers to the presence of B chromosomes.
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