In vitro plantlet regeneration from cotyledon, hypocotyl and root explants of hybrid seed geranium
1996
Chang, C. | Moll, B.A. | Evenson, K.B. | Guiltinan, M.J.
In vitro plantlet regeneration systems for the seed geranium (Pelargonium X hortorum Bailey) using cotyledon, hypocotyl and root explants were optimized by studying the influence of seedling age, growth regulators an excision orientation on organogenesis. Indole-3-acetic acid combined with zeatin yielded the highest rate of shoe production on cotyledon explants (0.2-2 shoots per explant). More shoots were produced on explants cut from the most basal region of cotyledons from 2 to 4-day-old seedlings than from older seedlings or more distal cut sites. Hypocotyl explants produced the highest number of shoots, up to 40 shoots per explant, on indole-3-acetic acid (2.8-5.6 mM) + zeatin (4.6 mM) or thidiazuron (4.5 mM). Maximum shoot formation (0.3-1.4 shoots per explant) on root explants occurred when they were cultured on medium containing zeatin. Regenerated shoots rooted best on a basal medium containing no growth regulators. There were substantial differences among cultivars in shoe formation from each of the explant systems.
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