Chemical composition, nutritional value and antioxidant properties of autochthonous Prunus avium cultivars from Campania Region
2014
Pacifico, Severina | Di Maro, Antimo | Petriccione, Milena | Galasso, Silvia | Piccolella, Simona | Di Giuseppe, Antonella M.A. | Scortichini, Marco | Monaco, Pietro
During a screening program aimed at the evaluation of antioxidative and antiproliferative properties, as well as nutritional properties of local edible plants, two endemic sweet cherry cultivars (‘Del Monte’ and ‘Della Recca’) were of interest. Macronutrient components (proteins, carbohydrates and lipids) of both the cherry cultivars were determined as well as free and total amino acids. Pomological traits were defined. HPLC–ESI/MSn analysis, carried out on phenolic extracts properly prepared by extractive techniques from freeze dried fruits of both the cherry cultivars, showed that investigated cultivars differed in their colorless phenolic composition. Hydroxycinnamoyl quinic acid derivatives were present in both the cherry cultivars. ‘Della Recca’ cv. was particularly rich in 4-O-coumaroyl quinic and 5-O-caffeoylquinic acid, whereas quercetin-3-O-rutinoside was the main phenol compound of ‘Del Monte’ cultivar.The antiradical properties of the extracts were investigated by DPPH and ABTS methods. ‘Della Recca’ cv. cherries exhibited a pronounced antiradical activity: at 62.5μg/mL dose level ABTS radical cation was converted in its reduced form by 88.7% and DPPH radical was reduced by 75.3%. The antiproliferative efficacy of ‘Della Recca’ and ‘Del Monte’ extracts were evaluated towards five cancer cell lines (HepG2, A549, HeLa, SK-B-NE(2)-C, and SH-SY5Y) through MTT assay. ‘Della Recca’ phenol extract showed a dose-dependent inhibiting activity towards cervical cancer HeLa cell line.
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Эту запись предоставил National Agricultural Library