Optimization of Saccharomyces boulardii production in solid-state fermentation with response surface methodology
Hu, Yuanliang | Qin, Huanhuan | Zhan, Zhichun | Dun, Yaohao | Zhou, Ying | Peng, Nan | Ling, Huayun | Liang, Samuel Y. | Zhao, Shumiao
Saccharomyces boulardii preparations are promising probiotics and clinical agents for animals and humans. This work focused on optimizing the nutritional conditions for the production of S. boulardii in solid-state fermentation by using classical and statistical methods. In single-factor experiments, the S. boulardii production was significantly increased by the addition of glucoamylase and the optimal carbon and nitrogen sources were found to be soluble starch and NH₄Cl, respectively. The effects of the glucoamylase, soluble starch and NH₄Cl on S. boulardii production were evaluated by a three-level three-factor Box–Behnken design and response surface methodology (RSM). The maximal yeast count (4.50 ×10⁹CFU/g) was obtained under the optimized conditions (198 U/g glucoamylase, 2.37% soluble starch and 0.9% NH₄Cl), which was in a good agreement with the predicted value of the model. This study has provided useful information on how to improve the accumulation of yeast cells by RSM.
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