Albino inflorescence proliferation of dendrocalamus latiflorus
2006
LIN, C. S. | TSENG, M. C. | HONG, P. I. | CHANG, W. C.
Inflorescence proliferation is a plant tissue culture technique that can be used to obtain in vitro inflorescences year-round without the intervening development of vegetative organs. In this study, we used albino mutant inflorescences of Dendrocalamus latiflorus as the original explant material to investigate the effect of plant growth regulators on long-term inflorescence proliferation. The albino inflorescences proliferated on solidified Murashige and Skoog (MS) basal medium supplemented with thidiazuron (TDZ), and the optimal concentration for successful long-term inflorescence proliferation was 0.45 μμM TDZ. A combination of αα-naphthaleneacetic acid (NAA) with 0.45 μμM TDZ inhibited the inflorescence proliferation. Inflorescences cultured on a TDZ-free medium supplemented with 26.82 μμM NAA rooted in 21 d, vegetative shoots formed by 42 d and, in one case, flowering occurred after 63 d. The auxins 2,4-dichlorophenoxyacetic acid (2,4-D, 4.52 μμM) and picloram (4.14 μμM) induced shoot formation. The protocol described can be used to produce large numbers of mutant inflorescences within a relatively short period of time.
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