Antibacterial Activity of Essential Oil and Extracts of Cleistocalyx operculatus Buds Against the Bacteria of Xanthomonas spp
2010
Bajpai, Vivek K. | Dung, Nguyen Thi | Suh, Hwa-Jin | Kang, S. C (Sun Chul)
This study was undertaken to assess the antibacterial efficacy of the essential oil and extracts of Cleistocalyx operculatus buds against plant pathogenic bacteria of Xanthomonas spp. The diameter of inhibition zones of oil (1,000 μg/disc) and extracts (1,500 μg/disc) against the tested bacteria were found in the range of 7-23 mm. The MIC and MBC values of the oil and extracts against the tested Xanthomonas spp. ranged from 31.25-125 to 62.5-250 μg/ml and 125-500 and 250-1,000 μg/ml, respectively. The cell viability study demonstrated a potential detrimental effect of the oil (1,000 μg/ml) and hexane extract (250 μg/ml) on the tested Xanthomonas spp. Also the oil displayed significant antibacterial effects in vivo against Xoo KX019 and Xsp SK12 conducted on greenhouse-grown oriental melon plants (Cucumis melo L. var. makuwa). The results of this study suggest that C. operculatus-derived essential oil and extracts could be used as natural bactericides in the food and agriculture industries.
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