Preparation of spent brewer's yeast beta-glucans with a potential application as an immunostimulant for black tiger shrimp, Penaeus monodon
2003
Suphantharika, M. | Khunrae, P. | Thanardkit, P. | Verduyn, C.
Spent brewer's yeast was autolysed and the insoluble yeast cell wall fraction obtained was used as a raw material for the preparation of brewer's yeast beta-glucan (BYG). A simple alkaline extraction was applied and optimized. The BYG with significantly (P < or = 0.05) high carbohydrate and glucan contents of 92% and 51% (w/w), respectively and with a low protein content of 1.6% (w/w) was obtained by a single alkaline extraction using 5 volumes of 1.0 N NaOH at 90 degrees C for 1 h. In vitro, this glucan significantly (P < or = 0.05) enhanced phenoloxidase (PO) activity of black tiger shrimp hemolymph as compared to controls without added glucan. Also in vivo, an oral administration of 0.2% (w/w) in diets for 3 days significantly (P < or = 0.05) increased the PO-activity of the shrimp. The potential immunostimulating properties of a commercial and other yeast-derived products were also evaluated for comparative purposes.
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