Evaluation of genetic diversity in plum germplasm collection using DNA-based S-genotyping
2013
Kota, I. | Lācis, G.
Plums belong to the Rosaceae family and are characterized by having the gametophytic self-incompatibility system, which is troublesome in fruit-growing. Successful yields can be obtained only by planting at least two pollen compatible cultivars in an orchard. Lack of information about pollen incompatibility among cultivars limits detailed characterization of germplasm, breeding efforts and appropriate orchard design. The plum germplasm collection at the Latvia State Institute of Fruit-Growing contains a wide diversity of plum accessions: domestic plum (P. domestica L.) cultivars of different origin, myrobalan (P. cerasifera L.) plums, as well as different interspecific hybrids involving P. cerasifera L., P. salicina Lindl. and P. Americana Marsh. S-genotyping was performed using consensus primers flanking the first and second intron of S-RNase gene and the SFB intron. Ninety-nine plum accessions were genotyped. All applied primers produced one or two bands per diploid plum cultivar, and one to six bands for hexaploid, domestic plums. In total 13 and 16 putative alleles were detected for the first and second S-RNase gene intron, respectively, and 7 alleles for SFB intron. No specific band patterns were discovered for particular plum species or cultivar groups indicating a common genetic basis of self-incompatibility among different plum species with various levels of ploidy. The S-genotyping data obtained can be used as an additional character for germplasm identification and in further research on the plum self-incompatibility system.
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