Trypanosoma cruzi Epimastigotes Express the Ouabain-and Vanadate-Sensitive (Na++K+)ATPase Activity
2014
Caruso-Neves, Celso | Einicker-Lamas, Marcelo | Chagas, Carlos | Oliveira, Mecia Maria | Vieyra, Adalberto | Lopes, Anibal Gil
The presence of (Na⁺+K⁺)ATPase activity in CL14 clone and NIH NTY strain of Trypanosoma cruzi epimastigotes is demonstrated. A Na⁺ plus K⁺ stimulated ATPase activity is found in both strains. The optimal Na⁺/K⁺ ratio is 5:1 and 9:1 in CL14 clone and NIH NTY strain, respectively. In both strains, vanadate completely inhibits the ouabain-sensitive ATPase activity indicating that it belongs to the P-type (E ₁/E₂) family of ion-transporting ATPases. The I₅₀ for vanadate is 0.66 ± 0.04 and 0.04 ± 0.02 μᴍ in CL14 clone and NIH NTY strain, respectively. These data indicate that both strains of T. cruzi epimastigotes express the oua bain-and vanadate-sensitive (Na⁺+K⁺)ATPase activity. On the other hand, the discrepancy between the parameters analyzed for the inhibitors suggests that they express different iso forms of this enzyme.
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