Coproduction of colicin V and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest
Langa, S. | Arqués, J.L. | Medina, M. | Landete, J.M.
AIMS: The aim of this study was the coproduction in a single strain of the Gram‐negative bacteriocin colicin V with other bacteriocins from lactic acid bacteria (LAB). METHODS AND RESULTS: Colicin V was expressed in Lactococcus and Enterococcus strains by replacing the colicin V leader peptide by the leader peptide and promoter of d‐alanyl‐d‐alanine carboxypeptidase from Lactobacillus reuteri CECT925 in pNZ8048 (pNZ:LR‐colV). The antimicrobial activity of colicin V against the indicator organism Escherichia coli DH5α in transformed strains was checked by agar diffusion assay and SDS‐PAGE analysis. CONCLUSIONS: Lactococcus and Enterococcus transformed with pNZ:LR‐colV were able to coproduce colicin V at high levels together with other LAB bacteriocins such as nisin A, nisin Z, lacticin 481 or enterocins A and B, obtaining broad‐spectrum activity strains with large potential applications. SIGNIFICANCE AND IMPACT OF THE STUDY: The construction showed in this work could be used for the heterologous expression of other bacteriocins active against Gram‐negative bacteria or wide‐spectrum bacteriocins from LAB.
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