Selection and characterization of a novel glyphosate-tolerant upland cotton (Gossypium hirsutum L.) mutant (R1098)
2010
Tong, X.H. | Daud, M.K. | Zhu, S.J.
With 2 figures and 4 tables Stepwise selection approach was adopted to obtain glyphosate-tolerant upland cotton mutant (R1098) from the embryogenic calli of 'Coker 312' (Gossypium hirsutum L.). The calli were transferred to the selection medium and multi-step selection pressure process was carried out until the calli could proliferate in the presence of 20 m m glyphosate. The regenerated plants from the glyphosate-tolerant calli were analysed for glyphosate response, tolerance of progenies and shikimate accumulation on whole-plant basis. 'R1098' could tolerate 1.48 kg acid equivalent/ha glyphosate. Progeny tests demonstrated that glyphosate tolerance trait was controlled by one dominant gene. Shikimate accumulation assay showed that significantly different amounts of shikimate were present between the mutant (R1098) and its wild parent (Coker 312) after glyphosate treatment. This indicates that 'R1098' has sufficient 5-enolpyruvylshikimate-3-phosphate synthase activity in the presence of glyphosate, thereby maintaining normal carbon flow through the shikimate pathway. Moreover, 'R1098' offers a greater potential to improve the weed control system in cotton production.
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