Total RNA isolation fromPicea mariana dry seed
2004
Tai, Helen H. | Pelletier, Céline | Beardmore, Tannis
Isolating RNA from dry conifer seeds can be difficult because of a number of interfering compounds present in seeds. We describe a protocol for total RNA isolation from black spruce dry seeds, which is an adaptation of a method used for mouse myeloma tissues. The extraction relies on selective precipitation of RNA by using lithium chloride.
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