Effect of ozone treatment on aflatoxin B1 and safety evaluation of ozonized corn
2014
Luo, Xiaohu | Wang, Ren | Wang, Li | Li, Yongfu | Bian, Yuanyuan | Chen, Zhengxing
This paper studies the ozone treatment effect on degradation of aflatoxin B1 (AFB1) in corn with different moisture content (MC). The toxicity of the degradation products (DPs) of the ozone-treated AFB1-Contaminated Corn (ACC) was also evaluated using the human hepatocellular carcinoma cell line (HepG2) as model cells. The degradation rate of AFB1 in corn increases with ozone concentration and treatment time. The results showed that ACC with 13.47% MC was easier to be degraded by ozone than with 20.37% MC. Treated with 90 mg L−1 ozone for 20 min and 40 min, AFB1 in corn with 13.47% MC decreased from 83 μg kg−1 to 18.12 μg kg−1 and 9.9 μg kg−1, respectively, well meeting the China National Standard of AFB1 in corn (20 μg kg−1). In order to evaluate the safety of ozone used on ACC, the impacts of AFB1 as well as untreated and ozone-treated ACC with the same level of AFB1 content on HepG2's survival rate, morphology, and apoptosis were studied. The results showed that ACC had high cell toxicity while the toxicity of ozone-treated ACC had no significant difference with that of the AFB1-free culture solution. It is concluded that ozonation can quickly and effectively degrade AFB1 in corn and diminish ACC's toxicity, and therefore, ozonation is expected to be an effective, fast, and safe method for AFB1 degradation in ACC.
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