Water extract of Brazilian green propolis attenuates high glucose-induced vascular morphological abnormality in zebrafish
Saitō, Yūichi | Gouda, Takumi | Nakamura, Shinsuke | Ichihara, Kenji | Hara, Hideaki | Shimazawa, Masamitsu
In diabetes patients, hyperglycemia causes many vascular disorders. To clarify the effects of water extract of Brazilian green propolis (WEP) against high glucose-induced vascular disorders, we investigated using zebrafish-based vasculopathy model. Zebrafish larvae, which is transparent and small, is an ideal model organism to detect changes of the vascular structure. Zebrafish larvae were exposed to 200 mM glucose and WEP solution from 2 h post fertilization (hpf) to 3 days post fertilization (dpf). The WEP treatment normalized high glucose-induced morphological abnormality of the larvae, hyaloid vessels, and cerebral vessels. The WEP treatment partly suppressed the elevation of glucose levels and mRNA levels of p27 in tissues by exposure to high glucose content. Furthermore, we found that chlorogenic acid, which is one of the main constituents of WEP, also normalized high glucose-induced morphological abnormality. Our findings indicated that WEP improves vascular integrity in the central nervous system under high glucose conditions.
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