Characterization of phospholipase D from Chorispora bungeana callus in response to freezing stress
Yang, N. | Yue, X. L. | Zhang, H. | Wu, G. F. | Ding, F. X. | Zhang, T. G. | An, L. Z.
The influence of freezing on phospholipase D (PLD) was studied in Chorispora bungeana Fisch. & C.A. Mey., which is a naturally cold-tolerant species. During the freezing treatment (−4 °C), PLD activities in both microsomal and mitochondrial membranes increased at day 3, remained at a high level at day 6 and then declined to a moderate level. The RT-PCR analyses showed that PLD activity partially corresponded to the CbPLD gene transcript level. The freezing treatment resulted in increases in the K ₘ and V ₘₐₓ for microsomal and mitochondrial PLD, respectively. Freezing injury, as measured by electrolyte leakage and malondialdehyde content, peaked at day 6 and then gradually decreased. Alleviation of freezing injury was related to a decreased content of membrane-associated Ca²⁺. We suggest that the specific mechanism of cold resistance of C. bungeana is linked with PLD.
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