Properties of enzyme modified corn, rice and tapioca starches
2009
Khatoon, Sakina | Sreerama, Y.N. | Raghavendra, D. | Bhattacharya, Suvendu | Bhat, K.K.
Corn, rice and tapioca starches were partially hydrolyzed by treating the starch dispersions with heat stable α-amylase. Dextrose equivalent (DE) of 8–12 was achieved by hydrolyzing the starch samples (10–20% w/v) for 30 min at 90 ± 2 °C. Scanning electron micrographs showed that starch granules had broken down to smaller particles. High performance liquid chromatography with refractive index detection indicated that oligosaccharides with broad molecular weight distributions are present in the reaction products. Hydrolyzed starch dispersions were analyzed for their rheological properties. The storage modulus values (G′) for 20% solid containing slurries were 7373 and 1470 Pa for untreated and enzyme treated samples, respectively, indicating a marked decrease in solid properties due to enzyme action. The complex viscosities (η*) for native corn starch and hydrolyzed corn starch were 8243 and 1637 Pas, respectively, which indicate that the enzyme treatment decreases the overall resistance of the sample to flow such that the product can spread easily. Further 13C CP/MAS NMR and FTIR studies revealed the loss of ordered structures in the enzyme modified starches. Free flowing fat substitute in the form of fine powder was prepared by spray drying the hydrolyzed starch slurry.
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