Substrate Concentration and Plasmid Transfer Frequency between Bacteria in a Model Rhizosphere
2000
Pearce, D. A | Bazin, M. J | Lynch, J. M
The influence of substrate concentration on plasmid transfer frequency in the rhizosphere was investigated using a physical model employing a hollow fiber membrane instead of a real root. The absolute number of transconjugants produced increased with increasing substrate (glucose) concentration, but the plasmid transfer frequency decreased exponentially with increasing substrate concentration from 4.4 × 10⁻³ at 90 μg ml⁻¹ glucose to 1.35 × 10⁻⁵ at 3600 μg ml⁻¹ glucose. These results were found to be heavily dependant on donor to recipient ratio and distribution of strains, but independent of initial donor and recipient inoculum density and regime. These observations also show that plasmid transfer frequency is reduced at high substrate concentrations, which suggests that plasmid transfer is either stimulated when growth limiting nutrient is scarce or inhibited when it is abundant.
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