Proteomic analysis of the phytogenic fungus Sclerotinia sclerotiorum
2020
Otun, Sarah | Ntushelo, Khayalethu
Sclerotinia sclerotiorum is a necrotrophic phytopathogen that has been the subject of several scientific research efforts. Despite the numerous research efforts its proteome remains understudied. This study aimed to identify proteins produced by S. sclerotiorum, thereby increasing the current proteomic knowledge base. Total proteins were extracted from mycelia scraped from five-day old cultures of S. sclerotiorum. The extracted proteins were separated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) and were annotated using the AB Sciex TripleToF 6600 mass spectrometer. Exactly 1471 proteins were reproducibly present in all three replicates. All proteins detected were classified based on their molecular and biological functions. To the knowledge of the authors, this is the most comprehensive proteomic study on S. sclerotiorum (judging by the high number of proteins identified).
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