Scale-up production of vitamin loaded heteroprotein coacervates and their protective property
Chapeau, Anne-Laure | Hamon, Pascaline | Rousseau, Florence | Croguennec, Thomas | Poncelet, Denis | Bouhallab, Saïd
The production of biocarriers for the protection and controlled delivery of bioactives is of great interest to develop functional foods. The ability of two whey proteins (WP), Beta-Lactoglobulin (BLG) and Lactoferrin (LF), to efficiently entrap vitamin B9 by complex coacervation has been reported at laboratory scale. In the present work, we report on the scaling-up production of B9 loaded BLG-LF coacervates (B9-WP coacervates). Complex coacervation was performed at bench scale, using commercial protein solutions. Under optimized conditions, B9-WP coacervates were produced by static mixing at a flow rate of 300 mL/min. Bench scale efficiency similar to that found for laboratory scale was reached with a coacervation yield of 65% and the B9 entrapment of 98%, demonstrating an efficient scaling-up. B9-WP coacervates showed good protection property for B9 during storage treatments, confirming the efficiency of this type of biocarrier for the development of natural functional foods.
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