A superficial phyto-assisted synthesis of silver nanoparticles and their assessment on hematological and biochemical parameters in Labeo rohita (Hamilton, 1822)
2013
Vignesh, Venkatasamy | Felix Anbarasi, Kulandaiyesu | Karthikeyeni, Sambhanthan | Sathiyanarayanan, Ganesan | Subramanian, Periyasamy | Thirumurugan, Ramasamy
Silver nanoparticles (AgNPs) were synthesized by phyto-reduction of silver nitrate (AgNO3) using aqueous leaf extract of Ocimum tenuiflorum (Tulasi). The biosynthesized AgNPs were characterized by UV–vis, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM). The critical analyzes confirmed that spherical and ovoid crystalline AgNPs with diameter of 7–15nm were synthesized and stabilized with plant extract as a layer. The colloidal solution of AgNPs showed significant antimicrobial activity against variety of human and fish pathogens in solid medium. The implications of colloidal AgNPs on hematological and biochemical functions of freshwater fish Labeo rohita were tested. The experiments revealed that the amount of hemoglobin and total count of blood cells were considerably increased. The activity of functional enzymes such as glutamic oxaloacetic transaminase (GOT), glutamate pyruvate transaminase (GPT), anti-protease and myloperoxidase were radically improved due to the treatment of fish with colloidal AgNPs and based on this study, it was found that the colloidal AgNPs having prominent immunomodulatory effects in fresh water fish. Therefore, the plant mediated synthesized colloidal AgNPs can be used as a “immunomodulator” in aquaculture sectors instead of synthetic growth factors.
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