Kojic acid, a potential inhibitor of NF-κB activation in transfectant human HaCaT and SCC-13 cells
2001
Moon, Ki-Young | Ahn, Kwang Seok | Lee, Jinseon | Kim, Yeong Shik
The activation of NF-κkB induced by kojic acid, an inhibitor of tyrosinase for biosynthesis of melanin in melanocytes, was investigated in human transfectant HaCaT and SCC-13 cells. These two keratinocyte cell lines transfected with pNF-κB-SEAP-NPT plasmid were used to determine the activation ofNF-KB. Transfectant cells release the secretory alkaline phosphatase (SEAP) as a transcription reporter in response to theNF-KB activity and contain the neomycin phosphotransferase (NPT) gene for the dominant selective marker of geneticin resistance. NF-κB activation was measured in the SEAP reporter gene assay using a fluorescence detection method. Kojic acid showed the inhibition of cellular NF-κB activity in both human keratinocyte transfectants. It could also downregulate the ultraviolet ray (UVR)-induced activation of NF-κB expression in transfectant HaCaT cells. Moreover, the inhibitory activity of kojic acid in transfectant HaCaT cells was found to be more potent than known antioxidants,e.g., vitamin C andN-acetyl-L-cysteine. These results indicate that kojic acid is a potential inhibitor of NF-κB activation in human keratinocytes, and suggest the hypothesis that NF-κB activation may be involved in kojic acid induced anti-melanogenic effect.
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