Quantification of bacteriophages ofBacteroides fragilis in environmental water samples of Seine River

Sun, Z. P.; Leṿi, Y.; Kiene, L.; Dumoutier, N.; Lucena, F.

Quantification of bacteriophages ofBacteroides fragilis in environmental water samples of Seine River

1997

Sun, Z. P. | Leṿi, Y. | Kiene, L. | Dumoutier, N. | Lucena, F.

Virus detection in drinking water is very important to protect human health. The different methodologies for analysing human pathogenic virus are very time consuming and expensive, so until now only a few specialised laboratories carried out this analysis. Detection of bacteriophages may be possible by examining the aquatic virus, with advantages of easy and cheap. The bacteriophages ofBacteroides fragilis have been proven as specifically present in human faeces and have relationships with water contaminated by enterovirus. Our study, for the first time in France, discovered theB. fragilis phages present in sample of sewage, Seine river and raw water for water supply. Our results also presented thatB. fragilis phages may be a better indicator for water bacteriology compared with classical bacteriological indicators in water treatment. On the other hand, our results demonstrated that MPN method (Most Probable Number) has more advantages than that of PFU (Plaque Forming Units).

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