A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters
2016
Bühligen, Franziska | Lucas, Rico | Nikolausz, Marcell | Kleinsteuber, Sabine
We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.
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