Differential reactivity of bovine lymphocytes to species of Brucella

Smith, R. III.; Kapatsa, J.C.; Sherwood, S.J. III.; Ficht, T.A.; Templeton, J.W.; Adams, L.G.

Differential reactivity of bovine lymphocytes to species of Brucella

1990

The reactivity of bovine lymphocytes to 4 species of Brucella was tested in thymidine-uptake assays, using long-term cultured lymphocytes and freshly obtained blood mononuclear cells. Lymphocytes were taken from cows that had been challenge exposed with a virulent strain of B abortus at midgestation. The cows were classified retrospectively as being naturally resistant or susceptible to brucellosis. Lymphocytes taken from these cows had 3 patterns of reactivity with species of Brucella: pattern 1 was defined by reactivity with 4 species (B abortus, B canis, B suis, and B melitensis); pattern 2 was defined by reactivity with all these species, except B melitensis; pattern 3 was defined by reactivity with B abortus and B canis, but not with B suis or B melitensis. There was a statistically significant correlation between susceptibility to brucellosis and expression of lymphocyte cross-reactivity with B suis (P < 0.01) and with B melitensis (P < 0.001).

Показать больше [+]