Sea Urchin Embryotoxicity Test for Environmental Contaminants—Potential Role of the MRP Proteins

Bošnjak, Ivana; Šegvić, Tanja; Smital, Tvrko; Franekić, Jasna; Mladineo, Ivona

Sea Urchin Embryotoxicity Test for Environmental Contaminants—Potential Role of the MRP Proteins

2011

Bošnjak, Ivana | Šegvić, Tanja | Smital, Tvrko | Franekić, Jasna | Mladineo, Ivona

The main goals of this study were (1) to standardize a simple and reliable embryotoxicity test for environmental contaminants and (2) to evaluate the presence and possible protective role of the multidrug resistance-associated (MRP) protein-mediated multixenobiotic defense in two Mediterranean sea urchin species, Paracentrotus lividus and Arbacia lixula. Toxic end-point used was the success of the first cell division in sea urchin embryos. Embryotoxicities of three environmentally relevant contaminants: mercuric chloride (HgCl2, 0.05–6 μM), trybutiltin (TBT, 2.5–500 nM), and oxybenzone (OXI, 0.1–100 μM); as well as seawater samples collected from the polluted and unpolluted locations, were determined and compared. A. lixula embryos were more sensitive to all three toxic compounds, and both P. lividus and A. lixula embryos were highly sensitive to TBT at nanomolar concentrations (EC50 49 ± 5 and 36.8 ± 3 nM, respectively). Inhibition of MRP protein by specific inhibitor MK571 caused significant increase in embryotoxic potency of HgCl2 (EC50 0.697 ± 0.03 and 0.245 ± 0.04 μM, respectively), TBT (EC50 24 ± 3 and 7.4 ± 1 nM, respectively) and polluted seawater sample, but not of OXI or unpolluted, natural seawater. Therefore, our results demonstrated for the first time the protective relevance of MRP proteins in early development of these two Mediterranean sea urchin species. Finally, the embryotoxicity protocol described in this study represents a simple and rapid bioassay for determination of environmentally relevant seawater contamination.

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