Simultaneous Determination of Paracetamol and Caffeine in Human Plasma by LC–ESI–MS
2008
Wang, Anna | Sun, Jin | Feng, Haijun | Gao, Shuo | He, Zhonggui
A rapid, selective and convenient liquid chromatography–mass spectrometric method for the simultaneous determination of paracetamol and caffeine in human plasma was developed and validated. Analytes and theophylline [internal standard (I.S.)] were extracted from plasma samples with diethyl ether-dichloromethane (3:2, v/v) and separated on a C₁₈ column (150 × 4.6 mm ID, 5 μm particle size, 100 Å pore size). The mobile phase consisted of 0.2% formic acid–methanol (60:40, v/v). The assay was linear in the concentration range between 0.05 and 25 μg mL⁻¹ for paracetamol and 10–5,000 ng mL⁻¹ for caffeine, with the lower limit of quantification of 0.05 μg mL⁻¹ and 10 ng mL⁻¹, respectively. The intra- and inter-day precision for both drugs was less than 8.1%, and the accuracy was within ±6.5%. The single chromatographic analysis of plasma samples was achieved within 4.5 min. This validated method was successfully applied to study the pharmacokinetics of paracetamol and caffeine in human plasma.
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