Response surface optimization of a glycolipid biosurfactant produced by a sponge associated marine bacterium Planococcus sp. MMD26
2019
Hema, T. | Seghal Kiran, G. | Sajayyan, Arya | Ravendran, Amrudha | Gowtham Raj, G. | Selvin, Joseph
Marine sponge associated bacterium Planococcus sp. MMD26 was isolated and selected for biosurfactant production. Criteria for screening of biosurfactant production by MMD26 were hemolytic activity, oil displacement test and drop collapsing test. Based on the response surface methodology (RSM)-based optimization experiments the biosurfactant production was enhanced to one fold over the wild strain. The biosurfactant produced by the strain Planococcus sp. MMD26 was characterized as a glycolipid biosurfactant based on FT-IR, GC-MS and 1H NMR spectra. The biosurfactant producing strain Planococcus sp. MMD26 had the ability to degrade 80% of different hydrocarbons present in the crude oil. The production of the glycolipid biosurfactant from Planococcus sp. MMD26 under submerged fermentation condtions revealed that the strain could be used for the pilot scale production of biosurfactant for environmental applications.
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