ADDITION OF SULFAMETHOXAZOLE TO SELECTIVE MEDIA AIDS IN THE RECOVERY OF CAMPYLOBACTER SPP. FROM BROILER RINSES
2008
LINE, J.E. | BAILEY, J.S. | BERRANG, M.E.
The association of Campylobacter spp. with raw poultry products has been well established. Campy-Line agar (CLA) is a recently developed selective culture medium that allows very few non-Campylobacter colonies to grow. The few contaminants able to grow on CLA from typical broiler chicken carcass rinses were identified and found to be sensitive to the antimicrobial agent sulfamethoxazole (SMX). The purpose of our experiment was to determine the influence of SMX on recovery of C. jejuni from broiler carcass rinse samples when added to Campy-Cefex agar or CLA. Cefex and CLA were prepared with and without the addition of 25 mg SMX/L media. Broiler carcass rinse samples (post-pick, n = 80 and post-chill, n = 80) were obtained from eight different commercial processors across the United States and were surface plated on the selective agars. Both Cefex and CLA with and without SMX recovered similar populations of Campylobacter; however, significantly fewer contaminants were observed on the Cefex with added SMX and the CLA with or without SMX compared to normal Cefex. The more selective CLA with SMX had no contaminants present from this sample type, which simplified enumeration. Addition of SMX should be considered for increasing selectivity of Campylobacter-specific media. The addition of sulfamethoxazole to Campylobacter-selective agar can reduce the number of contaminants on the agar and facilitate enumeration of Campylobacter colonies.
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