Identification and Quantification of Xanthophyll Esters, Carotenes, and Tocopherols in the Fruit of Seven Mexican Mango Cultivars by Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Time-of-Flight Mass Spectrometry [LC-(APcI+)-MS]
2007
Ornelas-Paz, J. de J. | Yahia, E.M. | Gardea-Bejar, A.
A liquid chromatography-mass spectrometry (LC-MS) method was developed to simultaneously identify and quantify carotenoids and tocopherols in the fruit of seven mango cultivars grown in Mexico. Fruit maturity was characterized objectively, and carotenoids and tocopherols were isolated by solvent extraction and analyzed by HPLC coupled to a C30 stationary phase and diode array, fluorescence, and mass (time-of-flight) detectors. All cultivars had a similar carotenoid pattern, in which all-trans-β-carotene and dibutyrates of all-trans-violaxanthin and 9-cis-violaxanthin were the most abundant. The content of all-trans-β-carotene ranged between 0.4 and 2.8 mg/100 g, and 'Haden' and 'Ataulfo' mangoes had the highest amount. The amounts of all-trans-violaxanthin and 9-cis-violaxanthin (as dibutyrates) ranged between 0.5 and 2.8 mg/100g and between 0.4 and 2.0 mg/100 g, respectively. The content of -tocopherol was low (200-500 microgram/100 g). The results of this study indicate that all-trans-β-carotene, all-trans-violaxanthin, and 9-cis-violaxanthin are the most abundant carotenoids in mango grown in Mexico.
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