Gene-centromere mapping in potato by half-tetrad analysis: map distances of H1, Rx, and Ry and their possible use for ascertaining the mode of 2n-pollen formation
1992
Wagenvoort, M. | Zimnoch-Guzowska, E.
Diploids from the tetraploid potato varieties 'Alcmaria' and 'Pansta' and from the tetraploid CPRO genotypes Y66-13-610 and Y66-13-636 were used in half-tetrad analyses to estimate the gene-centromere map distances of the genes Rx, Ry, and H1. Employing tetraploid progeny from 2x (second division restitution)--4x testcrosses the genecentromere map distance of H1, conferring resistance to pathotype Ro1 of Globodera rostochiensis, was estimated to be 16.3 centimorgans (cM). For Rx, conferring extreme resistance to potato virus X (PVX), a map distance of 33.9 cM was estimated. The gene Ry, conferring extreme resistance to potato virus Y (PVY), was estimated to be located 14.2 cM from the centromere. Using the estimated map distance for Rx, it was attempted to determine the mode of 2n-pollen formation in four diploid interspecific hybrids, including the species Solanum tuberosum, Solanum chacoense, Solanum yungasense, and Solanum phureja, by half-tetrad analysis in tetraploid progeny from 4x-2x testcrosses. The mean frequency of 8.7% nulliplex plants for Rx was outside the range of the 95% confidence intervals, for both first division restitution and second division restitution 2n pollen.
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