Biological control of Eutypa lata on grapevine by an antagonistic strain of Bacillus subtilis
Ferreira, J.H.S. | Matthee, F.N. | Thomas, A.C.
An isolate of Bacillus subtilis from grapevine wood inhibited in vitro growth of Eutypa lata, the causal organism of dieback in grapevines. On potato-dextrose agar, the bacterium caused 91.4% inhibition of mycelial growth of E. lata and 100% inhibition of ascospore germination. Ascospore germination correlated negatively with time of exposure to B. subtilis. Malformation of hyphae of E. lata occurred in the presence of B. subtilis. At a concentration of greater than or equal to 0.8 mg/ml, an antibiotic substance in an ethanol extract from B. subtilis totally inhibited germination of ascospores of E. lata. Thin-layer chromatography of crude antibiotic extract showed five bands, two of which inhibited mycelial growth of E. lata. Spraying a suspension of the bacterium on pruning wounds before inoculation with ascospores of E. lata significantly reduced infection as compared with the unsprayed, inoculated controls.
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