Effect of prostaglandin F2 alpha (PGF2 alpha) on sources of progesterone and pregnancy in intact, ovariectomized and hysterectomized 90-100 day pregnant ewes
Weems, Y.S. | Vincent, D.L. | Tanaka, Y. | Miller-Patrick, K. | Nusser, K.D. | Ashimine, D.T. | Ledgerwood, K. | Lee, C.N. | Weems, C.W.
A single dose of 8 or 16 mg of PGF 2-alpha per 58 kg body weight was injected intramuscular into intact, ovariectomized or hysterectomized 90-100 day pregnant sheep in three separate experiments. Both doses of PGF 2-alpha decreased the weights of the corpora lutea (P less than or equal to 0.05) and the concentration of progesterone in ovarian venous plasma at 72 hr (P less than or equal to 0.05) compared to the 0 hr sample within treatment groups and to control ewes at 72 hr in intact and hysterectomized pregnant ewes. In hysterectomized pregnant ewes, progesterone in jugular plasma declined (P less than or equal to 0.05) from 0 to 72 hr but never fell below 4 mg/ml and this decrease in progesterone after 8 or 16 mg PGF(2-alpha) was greater than in control hysterectomized ewes (P less than or equal to 0.05). There was a significant decrease in progesterone over time in jugular or uterine venous plasma in the presence of absence of the ovaries in 90-100 day pregnant ewes (P less than or equal to 0.05) but the profiles of progesterone were not different between vehicle and PGF 2-alpha-treated ewes (P greater than or equal to 0.05). Uterine venous progesterone never declined below 30 ng/ml in the presence or absence of the ovaries and there was a significant quadratic increase (P less than or equal to 0.05) in uterine venous progesterone toward the end of the 72 hr sampling period indicating an increase in steroidogenic activity of the placenta. PGF 2-alpha did not affect the number of abortions in intact or ovariectomized pregnant ewes (P > 0.05). Thus, the corpus luteum of sheep at 90-100 days of pregnancy is functional and responsive to PGF 2-alpha, placentomes are functional but do not appear to be responsive to the doses of PGF 2-alpha tested and PGF 2-alpha was not an abortifacient over the 72 hr treatment period.
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