Genotoxicity of sodium arsenite on Vicia faba root meristematic cells
2022
Kaur, Tajinder | Manish Kumar, | Kaur, Satwinderjeet
The present study was designed to evaluate the genotoxicity of sodium arsenite by employing the Vicia faba root chromosomal aberration assay. The seedlings were treated with different concentrations (0.2, 0.4, 0.6, 0.8, and 1 mg/l) of sodium arsenite. In addition to the cytogenetic assay, effect of arsenic on biochemical parameters such as total protein content and antioxidative enzymes [Sodium dismutase (SOD) and Guaiacol peroxidase (POD)] were also studied in 7-day old Vicia faba seedlings. Results demonstrated that metal stress significantly induced chromosomal aberrations and exhibited cytotoxicity by lowering the mitotic index from 9.37 ± 0.05 to 3.73 ± 0.01. Biochemical analysis showed a significant decrease in total protein content and increased activity of antioxidative enzymes under heavy metal stress as compared to control. The present investigations not only provide insights into arsenic genotoxicity but also support the use of various arsenic induced genotoxic characteristics as toxicity indicators in plants.
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