Effect of POU1F1, IGF1 and Leptin Genes Polymorphisms on Average Daily Gain in Makui Sheep Breed
Hosseinlou, Abbas Haji | Hashemi, Ali | Pirani, Nasrollah | Sadeqi, Saadat | Moqaddam, Farzaneh Fil Koush
For study polymorphism of POU1F1, IGF1 and Leptin genes and their relationships with daily gain, blood samples of 100 heads (65 males and 35 females) of Makui sheep breed were randomly collected. DNA was extracted from whole blood and polymerase chain reactions (PCR) were performed using three pairs of specific primers. Single Strand Conformation Polymorphism (SSCP) was used for detection of genotypes. Number of banding patterns (genotypes) for POU1F1, IGF1 and Leptin genes were 4, 3 and 5, respectively. A general linear model procedure was applied to determine association between genotypes with average daily gain in different stages. Genotypes of leptin, POU1F1 and IGF1 genes had significant effect (P<0.05) on the average daily gain. Banding patterns (genotypes) of AB and BB genotypes in IGF1 gene for average daily gain from birth to weaning (3 months) and average daily gain from 6 months to 9 months, respectively, CC and AA genotypes in POU1F1 gene for average daily gain from 6 months to 9 months and 9 months to yearling, respectively and BC genotype in Leptin gene for average daily gain from weaning to 6 months had higher performances than other genotypes. These results confirmed the potential usefulness of IGF1, POU1F1 and Leptin genes in marker-assisted selection programs in Makui breed.
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