Expeditious production of concentrated glucose-rich hydrolysate from sugarcane bagasse and its fermentation to lactic acid with high productivity
Baral, Pratibha | Pundir, Anushka | Kumar, Vinod | Kurmi, Akhilesh K. | Agrawal, Deepti
Sugarcane bagasse (SCB) is anticipated to emerge as a potential threat to waste management in India on account of cheap surplus energy options and lower incentives through its co-generation. Through biotechnological intervention, the efficient utilization of SCB is seen as an opportunity. The present study aimed towards expeditious production of concentrated glucose-rich hydrolysate from SCB. Alkali pretreated biomass was chosen for hydrolysis with a new generation cellulase cocktail, Cellic CTec2 dosed at 25 mg g⁻¹ glucan content. A two-step (9% + 9%) substrate feeding strategy was adopted with a gap of an hour, and saccharification was terminated in three different ways. Irrespective of the methods employed for termination, ∼84.5% cellulose was hydrolyzed releasing ≥100 g L⁻¹ glucose from 18% biomass. Direct use of glucose-rich filtrates yielded 69.2 ± 2.5 g L⁻¹ of L (+) lactic acid using thermophilic Bacillus coagulans NCIM 5648. The best-attained glucose and lactic acid productivities during separate hydrolysis and fermentation (SHF) in the present study were 5.27 and 2.88 g L⁻¹ h⁻¹, respectively. A green and sustainable process is demonstrated for the production of industrially relevant sugars from SCB at high productivity and its valorization to bio-based lactic acid.
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